Information about Biotechnology and related Biological Sciences applied in Healthcare sector and Agriculture
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DNA Finger Printing
DNA Fingerprinting
DNA fingerprinting is a method used to identify an individual from
a sample of DNA by looking at unique patterns in their DNA
The technique used to identify the individual that takes advantage of
differences in
DNA sequence. The process of DNA fingerprinting begins by isolating
DNA
from blood,
semen, vaginal fluids, hair roots, skin, skeletal remains, or
elsewhere. After we isolate the DNA and amplify it with PCR. Treat the DNA with
restriction enzymes e.g. cut DNA at specific sequences, because everyone’s DNA is
different, so everyone’s DNA will cut
at different sites. This results in different
sized fragments. The different sized fragments are called restriction fragment length
polymorphisms,
or RFLPs. We can observe the different sized fragments in an
experiment that separates DNA based on fragment size called Gel Electrophoresis.
Similar to RFLPs techniques, there are many other techniques uses in DNA
fingerprinting such as RAPD, SNP, AFLP etc. DNA fingerprints can be used to
determine which sample e.g. bone
fragments belong to which individual.
Sunday, April 22, 2018
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